Antibody fragments structurally enable a drug-discovery campaign on the cancer target Mcl-1.
Acta Crystallogr D Struct Biol
; 75(Pt 11): 1003-1014, 2019 Nov 01.
Article
en En
| MEDLINE
| ID: mdl-31692474
ABSTRACT
Apoptosis is a crucial process by which multicellular organisms control tissue growth, removal and inflammation. Disruption of the normal apoptotic function is often observed in cancer, where cell death is avoided by the overexpression of anti-apoptotic proteins of the Bcl-2 (B-cell lymphoma 2) family, including Mcl-1 (myeloid cell leukaemia 1). This makes Mcl-1 a potential target for drug therapy, through which normal apoptosis may be restored by inhibiting the protective function of Mcl-1. Here, the discovery and biophysical properties of an anti-Mcl-1 antibody fragment are described and the utility of both the scFv and Fab are demonstrated in generating an Mcl-1 crystal system amenable to iterative structure-guided drug design.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
/
6_ODS3_enfermedades_notrasmisibles
Problema de salud:
3_neglected_diseases
/
3_zoonosis
/
6_ovary_cancer
Asunto principal:
Fragmentos Fab de Inmunoglobulinas
/
Descubrimiento de Drogas
/
Anticuerpos de Cadena Única
/
Proteína 1 de la Secuencia de Leucemia de Células Mieloides
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Acta Crystallogr D Struct Biol
Año:
2019
Tipo del documento:
Article
País de afiliación:
Reino Unido