Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum.
J Vis Exp
; (152)2019 10 26.
Article
en En
| MEDLINE
| ID: mdl-31710031
ABSTRACT
The use of primary cell cultures has become one of the major tools to study the nervous system in vitro. The ultimate goal of using this simplified model system is to provide a controlled microenvironment and maintain the high survival rate and the natural features of dissociated neuronal and nonneuronal cells as much as possible under in vitro conditions. In this article, we demonstrate a method of isolating primary neurons from the developing mouse cerebellum, placing them in an in vitro environment, establishing their growth, and monitoring their viability and differentiation for several weeks. This method is applicable to embryonic neurons dissociated from cerebellum between embryonic days 12-18.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cerebelo
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
J Vis Exp
Año:
2019
Tipo del documento:
Article