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Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum.
Shabanipour, Shahin; Dalvand, Azadeh; Jiao, Xiaodan; Rahimi Balaei, Maryam; Chung, Seung H; Kong, Jiming; Del Bigio, Marc R; Marzban, Hassan.
Afiliación
  • Shabanipour S; Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba; The Children's Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba.
  • Dalvand A; Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba; The Children's Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba.
  • Jiao X; Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba; The Children's Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba.
  • Rahimi Balaei M; Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba; The Children's Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba.
  • Chung SH; Department of Oral Biology, University of Illinois at Chicago.
  • Kong J; Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba.
  • Del Bigio MR; The Children's Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba; Department of Pathology, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba.
  • Marzban H; Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba; The Children's Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba; hassan.marzban@u
J Vis Exp ; (152)2019 10 26.
Article en En | MEDLINE | ID: mdl-31710031
ABSTRACT
The use of primary cell cultures has become one of the major tools to study the nervous system in vitro. The ultimate goal of using this simplified model system is to provide a controlled microenvironment and maintain the high survival rate and the natural features of dissociated neuronal and nonneuronal cells as much as possible under in vitro conditions. In this article, we demonstrate a method of isolating primary neurons from the developing mouse cerebellum, placing them in an in vitro environment, establishing their growth, and monitoring their viability and differentiation for several weeks. This method is applicable to embryonic neurons dissociated from cerebellum between embryonic days 12-18.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cerebelo / Neuronas Límite: Animals Idioma: En Revista: J Vis Exp Año: 2019 Tipo del documento: Article
Texto completo
Añadir a Mi BVS
Imprimir
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cerebelo / Neuronas Límite: Animals Idioma: En Revista: J Vis Exp Año: 2019 Tipo del documento: Article