Salt Response Analysis in Two Rice Cultivars at Seedling Stage.

ABSTRACT

In order to explore the salt-stress responses of two rice varieties, the physiological responses and biochemical responses were investigated using proteomics and classical biochemical methods. The results showed that the seedling growth was inhibited under salt condition in two rice varieties, the seedling growth in the tolerant variety was better than the sensitive variety. The sensitive variety(L7) appeared obvious salt-injury under 3-day salt stress, the tolerant variety (T07339) keep normal growth under 7-day salt stress except that the shoot length was decreased. Through the growth-parameters analysis, most of them in L7 were restrained by salinity and most in T07339 were unaffected. In T07339, the fresh root weight, the content of chlorophyll and the fresh shoot weight were even increased after 7 days of salt stress. A comparison of two-dimensional gel electrophoresis (2-DGE) protein profiles revealed 8 differently expressed proteins. Four proteins were expressed in different pattern between sensitive and tolerant varieties. These results provide novel insights into the investigations of the salt-response proteins that involved in improved salt tolerance.