p-Aminobenzoic acid as an alternative chemical biopsy agent for human hepatic UDP-glucose.
Anal Biochem
; 590: 113511, 2020 02 01.
Article
en En
| MEDLINE
| ID: mdl-31759975
ABSTRACT
p-Aminobenzoic acid (PABA) was evaluated for noninvasive sampling of UDP-glucose in the liver. Six healthy subjects ingested 550 mg PABA during a breakfast meal. Urine was collected 0-2 and 2-4 h after PABA ingestion. N-acetyl PABA glucuronide (NAPG) was identified with 522 ± 212 µmol recovered in the 2-4 h urines. One of the subjects ingested 2 g of 98% [U-2H7]glucose alongside PABA and the NAPG was analyzed for positional 2H-enrichment by 2H NMR following derivatization to 5-O-acetyl monoacetone glucuronolactone. In conclusion, PABA is an effective agent for the chemical biopsy of hepatic UDP-glucose in humans.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Uridina Difosfato Glucosa
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Biopsia
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Ácido 4-Aminobenzoico
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Hígado
Límite:
Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Anal Biochem
Año:
2020
Tipo del documento:
Article
País de afiliación:
Portugal