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A Rare Thioquinolobactin Siderophore Present in a Bioactive Pseudomonas sp. DTU12.1.
Sazinas, Pavelas; Hansen, Morten Lindqvist; Aune, May Iren; Fischer, Marie Højmark; Jelsbak, Lars.
Afiliación
  • Sazinas P; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.
  • Hansen ML; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.
  • Aune MI; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.
  • Fischer MH; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.
  • Jelsbak L; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.
Genome Biol Evol ; 11(12): 3529-3533, 2019 12 01.
Article en En | MEDLINE | ID: mdl-31800028
ABSTRACT
Many of the soil-dwelling Pseudomonas species are known to produce secondary metabolite compounds, which can have antagonistic activity against other microorganisms, including important plant pathogens. It is thus of importance to isolate new strains of Pseudomonas and discover novel or rare gene clusters encoding bioactive products. In an effort to accomplish this, we have isolated a bioactive Pseudomonas strain DTU12.1 from leaf-covered soil in Denmark. Following genome sequencing with Illumina and Oxford Nanopore technologies, we generated a complete genome sequence with the length of 5,943,629 base pairs. The DTU12.1 strain contained a complete gene cluster for a rare thioquinolobactin siderophore, which was previously described as possessing bioactivity against oomycetes and several fungal species. We placed the DTU12.1 strain within Pseudomonas gessardii subgroup of fluorescent pseudomonads, where it formed a distinct clade with other Pseudomonas strains, most of which also contained a complete thioquinolobactin gene cluster. Only two other Pseudomonas strains were found to contain the gene cluster, though they were present in a different phylogenetic clade and were missing a transcriptional regulator of the whole cluster. We show that having the complete genome sequence and establishing phylogenetic relationships with other strains can enable us to start evaluating the distribution and evolutionary origins of secondary metabolite clusters.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas / Quinolinas / Vías Biosintéticas Idioma: En Revista: Genome Biol Evol Asunto de la revista: BIOLOGIA / BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article País de afiliación: Dinamarca

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas / Quinolinas / Vías Biosintéticas Idioma: En Revista: Genome Biol Evol Asunto de la revista: BIOLOGIA / BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article País de afiliación: Dinamarca
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