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Localization microscopy at doubled precision with patterned illumination.
Cnossen, Jelmer; Hinsdale, Taylor; Thorsen, Rasmus Ø; Siemons, Marijn; Schueder, Florian; Jungmann, Ralf; Smith, Carlas S; Rieger, Bernd; Stallinga, Sjoerd.
Afiliación
  • Cnossen J; Department of Imaging Physics, Delft University of Technology, Delft, the Netherlands.
  • Hinsdale T; Delft Center for Systems and Control, Delft University of Technology, Delft, the Netherlands.
  • Thorsen RØ; Department of Imaging Physics, Delft University of Technology, Delft, the Netherlands.
  • Siemons M; Department of Imaging Physics, Delft University of Technology, Delft, the Netherlands.
  • Schueder F; Department of Biology, University of Utrecht, Utrecht, the Netherlands.
  • Jungmann R; Department of Physics and Center for Nanoscience, Ludwig Maximilian University, Munich, Germany.
  • Smith CS; Max Planck Institute of Biochemistry, Martinsried, Germany.
  • Rieger B; Department of Physics and Center for Nanoscience, Ludwig Maximilian University, Munich, Germany.
  • Stallinga S; Max Planck Institute of Biochemistry, Martinsried, Germany.
Nat Methods ; 17(1): 59-63, 2020 01.
Article en En | MEDLINE | ID: mdl-31819263
ABSTRACT
MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Iluminación / ADN / Nanoestructuras / Imagen Individual de Molécula / Microscopía Fluorescente / Modelos Teóricos Límite: Animals / Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2020 Tipo del documento: Article País de afiliación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Iluminación / ADN / Nanoestructuras / Imagen Individual de Molécula / Microscopía Fluorescente / Modelos Teóricos Límite: Animals / Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2020 Tipo del documento: Article País de afiliación: Países Bajos