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Magnetic resonance measurements of cellular and sub-cellular membrane structures in live and fixed neural tissue.
Williamson, Nathan H; Ravin, Rea; Benjamini, Dan; Merkle, Hellmut; Falgairolle, Melanie; O'Donovan, Michael James; Blivis, Dvir; Ide, Dave; Cai, Teddy X; Ghorashi, Nima S; Bai, Ruiliang; Basser, Peter J.
Afiliación
  • Williamson NH; Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States.
  • Ravin R; Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States.
  • Benjamini D; Celoptics, Rockville, United States.
  • Merkle H; Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States.
  • Falgairolle M; Center for Neuroscience and Regenerative Medicine, Henry Jackson Foundation, Bethesda, United States.
  • O'Donovan MJ; National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States.
  • Blivis D; National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States.
  • Ide D; National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States.
  • Cai TX; National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States.
  • Ghorashi NS; National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States.
  • Bai R; National Institute of Mental Health, National Institutes of Health, Bethesda, United States.
  • Basser PJ; Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States.
Elife ; 82019 12 12.
Article en En | MEDLINE | ID: mdl-31829935
We develop magnetic resonance (MR) methods for real-time measurement of tissue microstructure and membrane permeability of live and fixed excised neonatal mouse spinal cords. Diffusion and exchange MR measurements are performed using the strong static gradient produced by a single-sided permanent magnet. Using tissue delipidation methods, we show that water diffusion is restricted solely by lipid membranes. Most of the diffusion signal can be assigned to water in tissue which is far from membranes. The remaining 25% can be assigned to water restricted on length scales of roughly a micron or less, near or within membrane structures at the cellular, organelle, and vesicle levels. Diffusion exchange spectroscopy measures water exchanging between membrane structures and free environments at 100 s-1.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Médula Espinal / Espectroscopía de Resonancia Magnética / Membrana Celular / Imagen de Difusión por Resonancia Magnética / Membranas Intracelulares Límite: Animals Idioma: En Revista: Elife Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Médula Espinal / Espectroscopía de Resonancia Magnética / Membrana Celular / Imagen de Difusión por Resonancia Magnética / Membranas Intracelulares Límite: Animals Idioma: En Revista: Elife Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos
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