Determination of chlorhexidine retention in different oral sites using matrix-assisted laser desorption/ionization-time of flight mass spectrometry.
Arch Oral Biol
; 110: 104623, 2020 Feb.
Article
en En
| MEDLINE
| ID: mdl-31830639
ABSTRACT
OBJECTIVE:
The aim of this study was to determine chlorhexidine retention in different oral sites after a one-time 30 s mouth rinsing.DESIGN:
Five volunteers were asked to rinse their mouth with 10 ml of 0.2 % chlorhexidine digluconate for 30 s. After rinsing, samples were collected from the interdental area, buccal dental pellicle, anterior labial and posterior buccal mucosa, and saliva with a microbrush at five-time points within 24 h. Retention of chlorhexidine was measured using matrix-assisted laser desorption/ionization-time of flight mass spectrometry with a quantification limit of 15 ng/ml.RESULTS:
Chlorhexidine remained in the oral cavity at micrograms per milliliter levels for 11 h after mouth rinsing and was even detected 24 h after application. The results showed a distinct decline of intraoral chlorhexidine levels during the first 6 h after rinsing and it was then retained at low concentrations for at least 24 h.CONCLUSIONS:
The dental pellicle and oral mucosa were favorable sites for chlorhexidine retention. The novel method used for chlorhexidine determination offered excellent quantification limits and readily permitted quantification of chlorhexidine.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Clorhexidina
/
Desinfectantes
/
Boca
/
Antisépticos Bucales
Límite:
Humans
Idioma:
En
Revista:
Arch Oral Biol
Año:
2020
Tipo del documento:
Article