Microrheology, microcirculation and structural compensatory mechanisms of a chronic kidney disease rat model. A preliminary study.
Clin Hemorheol Microcirc
; 75(1): 47-56, 2020.
Article
en En
| MEDLINE
| ID: mdl-31884459
ABSTRACT
BACKGROUND:
Chronic kidney disease (CKD) models are known to study pathophysiology and various treatment methods. Renal dysfunction could influence erythrocytes through several pathways. However, hemorheological and microcirculatory relation of CKD models are not completely studied yet.OBJECTIVE:
To evaluate erythrocyte micro-rheology, microcirculatory and structural compensatory mechanisms in a rat model of CKD.METHODS:
Female Sprague-Dawley rats were subjected to nephrectomy group (NG, nâ=â6) or sham-operated group (SG, nâ=â6). NG rats were subjected to 5/6 nephrectomy in two stages. In SG no intervention was made on kidneys. Hemorheological and hematological measurements were carried out after each stage, and 5 weeks after the last operation. Histological and microcirculatory studies were done on the remaining kidney and compared with sham rats.RESULTS:
Serum creatinine increased in NG (pâ=â0.008), accompanied with decrease of red blood cell count (pâ=â0.028) and hemoglobin (pâ=â0.015). Erythrocyte aggregation parameters slightly increased in NG, while the elongation index didn't show significant changes. Microcirculation was intact in the remnant kidney of NG. However, in comparison with SG, the diameter of glomeruli increased significantly (pâ<â0.01).CONCLUSIONS:
Erythrocyte mass was influenced more than micro-rheological properties in this model. The main compensation mechanism was rather structural than at microcirculatory level.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Reología
/
Insuficiencia Renal Crónica
/
Microcirculación
Límite:
Animals
Idioma:
En
Revista:
Clin Hemorheol Microcirc
Asunto de la revista:
ANGIOLOGIA
/
HEMATOLOGIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Hungria