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Enzymatic Synthesis of Anabolic Steroid Glycosides by Glucosyltransferase from Terribacillus sp. PAMC 23288.
Yu, Eun-Ji; Yamaguchi, Tokutaro; Lee, Joo-Ho; Lim, A-Rang; Lee, Jun Hyuck; Park, Hyun; Oh, Tae-Jin.
Afiliación
  • Yu EJ; Department of Life Science and Biochemical Engineering, SunMoon University, Asan 31460, Republic of Korea.
  • Yamaguchi T; Department of Life Science and Biochemical Engineering, SunMoon University, Asan 31460, Republic of Korea.
  • Lee JH; Department of Pharmaceutical Engineering and Biotechnology, SunMoon University, Asan 31460, Republic of Korea.
  • Lim AR; Genome-Based BioIT Convergence Institute, Asan 31460, Republic of Korea.
  • Lee JH; Genome-Based BioIT Convergence Institute, Asan 31460, Republic of Korea.
  • Park H; Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea.
  • Oh TJ; Unit of Research for Practical Application, Korea Polar Research Institute, Incheon 21990, Republic of Korea.
J Microbiol Biotechnol ; 30(4): 604-614, 2020 Apr 28.
Article en En | MEDLINE | ID: mdl-31893610
The application of steroids has steadily increased thanks to their therapeutic effects. However, alternatives are required due their severe side effects; thus, studies on the activities of steroid derivatives are underway. Sugar derivatives of nandrolone, which is used to treat breast cancer, as well as cortisone and prednisone, which reduce inflammation, pain, and edema, are unknown. We linked O-glucose to nandrolone and testosterone using UDP-glucosyltransferase (UGT-1) and, then, tested their bioactivities in vitro. Analysis by NMR showed that the derivatives were 17ß-nandrolone ß-D-glucose and 17ß-testosterone ß-D-glucose, respectively. The viability was higher and cytotoxicity was evident in PC12 cells incubated with rotenone and, testosterone derivatives, compared to the controls. SH-SY5Y cells incubated with H2O2 and nandrolone derivatives remained viable and cytotoxicity was attenuated. Both derivatives enhanced neuronal protective effects and increased the amounts of cellular ATP.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillaceae / Congéneres de la Testosterona / Glucosiltransferasas / Glicósidos Límite: Animals / Humans Idioma: En Revista: J Microbiol Biotechnol Año: 2020 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillaceae / Congéneres de la Testosterona / Glucosiltransferasas / Glicósidos Límite: Animals / Humans Idioma: En Revista: J Microbiol Biotechnol Año: 2020 Tipo del documento: Article
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