Mechanisms of Acquired <i>In Vivo</i> and <i>In Vitro</i> Resistance to Voriconazole by Candida krusei following Exposure to Suboptimal Drug Concentration.

Ricardo, Elisabete; Grenouillet, Fréderic; Miranda, Isabel M; Silva, Raquel M; Eglin, Guilluame; Devillard, Nadège; Rodrigues, Acácio Gonçalves; Pina-Vaz, Cidália

Mechanisms of Acquired In Vivo and In Vitro Resistance to Voriconazole by Candida krusei following Exposure to Suboptimal Drug Concentration.

Ricardo, Elisabete; Grenouillet, Fréderic; Miranda, Isabel M; Silva, Raquel M; Eglin, Guilluame; Devillard, Nadège; Rodrigues, Acácio Gonçalves; Pina-Vaz, Cidália.

Afiliación

Ricardo E; Division of Microbiology, Department of Pathology, Faculty of Medicine, University of Porto, Porto, Portugal ericardo@med.up.pt.
Grenouillet F; CINTESIS - Center for Health Technology and Services Research, Faculty of Medicine, University of Porto, Porto, Portugal.
Miranda IM; Medical Biology Department, University Hospital, Besançon, France.
Silva RM; UMR UBFC-CNRS 6249 ChronoEnvironnement, University of Bourgogne-Franche Comte, Besançon, France.
Eglin G; Division of Microbiology, Department of Pathology, Faculty of Medicine, University of Porto, Porto, Portugal.
Devillard N; Cardiovascular R&D Unit, Faculty of Medicine of the University of Porto, Porto, Portugal.
Rodrigues AG; Department of Medical Sciences and iBiMED, University of Aveiro, Aveiro, Portugal.
Pina-Vaz C; Medical Biology Department, University Hospital, Besançon, France.

Antimicrob Agents Chemother ; 64(4)2020 03 24.

Article en En | MEDLINE | ID: mdl-31932372

ABSTRACT

ABSTRACT

Five Candida krusei isolates (susceptible and resistant) recovered from the urine of a kidney transplant patient treated with voriconazole (VRC) 200 mg twice daily for 20 days were studied. Eight unrelated clinical isolates of C. krusei were exposed in vitro to VRC 0.001 µg/ml for 30 days. Development of VRC transient resistance occurred in vivo, and induction of permanent resistance occurred in vitro Mostly, ABC1 and ERG11 genes were overexpressed, and a homozygous T418C mutation in the ERG11 gene was found.

Asunto(s)

Adaptación Fisiológica/efectos de los fármacos; Antifúngicos/farmacología; Farmacorresistencia Fúngica/genética; Micosis/tratamiento farmacológico; Pichia/efectos de los fármacos; Voriconazol/farmacología; Transportadoras de Casetes de Unión a ATP/genética; Sistema Enzimático del Citocromo P-450/genética; Femenino; Humanos; Trasplante de Riñón/efectos adversos; Pruebas de Sensibilidad Microbiana; Micosis/microbiología; Pichia/genética; Pichia/aislamiento & purificación; Adulto Joven

Palabras clave

ABC1 ABC2 efflux pump genes; Candida krusei; ERG11 gene mutations; candidiasis; candiduria; mechanisms of antifungal resistance; underexposure to antifungal drugs; voriconazole

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 3_ND Problema de salud: 3_leprosy Asunto principal: Pichia / Adaptación Fisiológica / Farmacorresistencia Fúngica / Voriconazol / Micosis / Antifúngicos Límite: Adult / Female / Humans Idioma: En Revista: Antimicrob Agents Chemother Año: 2020 Tipo del documento: Article País de afiliación: Portugal

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