Implementation of a Multiplex rRT-PCR for Zika, Chikungunya, and Dengue Viruses: Improving Arboviral Detection in an Endemic Region.
Am J Trop Med Hyg
; 102(3): 625-628, 2020 03.
Article
en En
| MEDLINE
| ID: mdl-31933462
ABSTRACT
Arboviral diagnosis has been complicated throughout the tropical and subtropical Americas by the recent co-circulation of Zika virus (ZIKV), chikungunya virus (CHIKV), and dengue virus (DENV). The aim of this study was to implement a multiplex real-time RT-PCR (rRT-PCR) for ZIKV, CHIKV, and DENV in Paraguay to test patients who were clinically suspected of having dengue. We tested 110 sera from patients who presented to the Hospital de Clínicas in 2016 and had testing for DENV nonstructural protein 1 (NS1; 40 positive and 70 negative). Using a composite reference standard, we confirmed 51 dengue cases (46.4%) 38/40 NS1 positive and 13/70 NS1 negative. Chikungunya virus and ZIKV were detected in one sample each, both were DENV NS1 negative. The NS1 test demonstrated good agreement with rRT-PCR for DENV. However, multiplex rRT-PCR identified a subset of dengue cases and additional arboviral infections that would not be detected if NS1 assays are relied upon for diagnosis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
2_ODS3
/
3_ND
Problema de salud:
2_enfermedades_transmissibles
/
3_chikungunya
/
3_dengue
/
3_neglected_diseases
Asunto principal:
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Dengue
/
Reacción en Cadena de la Polimerasa Multiplex
/
Fiebre Chikungunya
/
Infección por el Virus Zika
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
/
Middle aged
País/Región como asunto:
America do sul
/
Paraguay
Idioma:
En
Revista:
Am J Trop Med Hyg
Año:
2020
Tipo del documento:
Article
País de afiliación:
Paraguay