Direct comparison study between droplet digital PCR and a combination of allele-specific PCR, asymmetric rapid PCR and melting curve analysis for the detection of BRAF V600E mutation in plasma from melanoma patients.

Tzanikou, Eleni; Haselmann, Verena; Markou, Athina; Duda, Angelika; Utikal, Jochen; Neumaier, Michael; Lianidou, Evi S

Tzanikou, Eleni; Haselmann, Verena; Markou, Athina; Duda, Angelika; Utikal, Jochen; Neumaier, Michael; Lianidou, Evi S.

Afiliación

Tzanikou E; Analysis of Circulating Tumor Cells Lab, Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, Greece.
Haselmann V; Institute for Clinical Chemistry, University Medical Center, Ruprecht-Karls University of Heidelberg, Mannheim, Germany.
Markou A; Analysis of Circulating Tumor Cells Lab, Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, Greece.
Duda A; Institute for Clinical Chemistry, University Medical Center, Ruprecht-Karls University of Heidelberg, Mannheim, Germany.
Utikal J; Department of Dermatology, Venerology and Allergology, University Medical Center, Ruprecht-Karls University of Heidelberg, Mannheim, Germany.
Neumaier M; Skin Cancer Unit, German Cancer Research Center (DKFZ), Heidelberg, Germany.
Lianidou ES; Institute for Clinical Chemistry, University Medical Center, Ruprecht-Karls University of Heidelberg, Mannheim, Germany.

Clin Chem Lab Med ; 58(11): 1799-1807, 2020 10 25.

Article en En | MEDLINE | ID: mdl-31953992

Asunto(s)

ADN Tumoral Circulante/sangre; Melanoma/sangre; Proteínas Proto-Oncogénicas B-raf/sangre; Adulto; Anciano; Anciano de 80 o más Años; Estudios de Cohortes; Femenino; Humanos; Biopsia Líquida; Masculino; Melanoma/genética; Persona de Mediana Edad; Mutación; Reacción en Cadena de la Polimerasa/métodos; Proteínas Proto-Oncogénicas B-raf/genética; Manejo de Especímenes/métodos; Factores de Tiempo

Palabras clave

BRAF mutation; ctDNA analysis; liquid biopsy; melanoma; plasma

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Proto-Oncogénicas B-raf / ADN Tumoral Circulante / Melanoma Tipo de estudio: Diagnostic_studies / Etiology_studies / Incidence_studies / Observational_studies / Risk_factors_studies Límite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Clin Chem Lab Med Asunto de la revista: QUIMICA CLINICA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2020 Tipo del documento: Article País de afiliación: Grecia

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