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Parallel Detection of Fundamental and Sixth Harmonic Signals Using an ICR Cell with Dipole and Sixth Harmonic Detectors.
Park, Sung-Gun; Anderson, Gordon A; Bruce, James E.
Afiliación
  • Park SG; Department of Genome Sciences, University of Washington, Seattle, Washington 98109, United States.
  • Anderson GA; GAA Custom Engineering, LLC, Benton City, Washington 99320, United States.
  • Bruce JE; Department of Genome Sciences, University of Washington, Seattle, Washington 98109, United States.
J Am Soc Mass Spectrom ; 31(3): 719-726, 2020 Mar 04.
Article en En | MEDLINE | ID: mdl-31967815
ABSTRACT
Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) is a powerful instrument for high-resolution analysis of biomolecules. However, relatively long signal acquisition periods are needed to achieve mass spectra with high resolution. The use of multiple detector electrodes for detection of harmonic frequencies has been introduced as one approach to increase scan rate for a given resolving power or to obtain increased resolving power for a given detection period. The achieved resolving power and scan rate increase linearly with the order of detected harmonic signals. In recent years, ICR cell geometries have been investigated to increase the order of the harmonic frequencies and enhance harmonic signal intensities. In this study, we demonstrated PCB-based ICR cell designs with dipole and sixth harmonic detectors for parallel detection of fundamental and harmonic (6f) signals. The sixth harmonic signals from the sixth harmonic detector showed an expected 6 times higher resolving power with (M + 3H)3+ charge state insulin ions as compared with that from fundamental signals from the dipole detector. Moreover, the insulin isotopic peaks with sixth harmonic frequency signals acquired with the sixth harmonic detector were resolved for a 40 ms data acquisition period but unresolved with the same duration dipole detector signals, corresponding to a 6-fold improvement in achievable spectral acquisition rates for a given resolving power.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Am Soc Mass Spectrom Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Am Soc Mass Spectrom Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos
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