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Cellular, physiological and molecular approaches to investigate the antifungal and anti-aflatoxigenic effects of thyme essential oil on Aspergillus flavus.
Oliveira, Rodrigo C; Carvajal-Moreno, Magda; Correa, Benedito; Rojo-Callejas, Francisco.
Afiliación
  • Oliveira RC; Laboratorio de Micotoxinas, Departamento de Botánica, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico.
  • Carvajal-Moreno M; Laboratorio de Micotoxinas, Departamento de Botánica, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico. Electronic address: magdac@ib.unam.mx.
  • Correa B; Laboratory of Mycotoxins and Mycotoxigenic Fungi, Department of Microbiology, University of Sao Paulo, Sao Paulo, Brazil.
  • Rojo-Callejas F; Facultad de Química, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico.
Food Chem ; 315: 126096, 2020 Jun 15.
Article en En | MEDLINE | ID: mdl-31991259
Several approaches, including the detection of apoptotic-like cell death, aflatoxin B1 (AFB1) production and gene expression analysis, were carried out to provide insights into the antifungal and anti-aflatoxigenic effects of thyme essential oil (EO) on Aspergillus flavus. At 0.5 µL mL-1, thyme EO completely inhibited A. flavus growth. Furthermore, this antifungal activity triggered significant apoptosis, via nuclear condensation (87.5% of nuclei analyzed) and plasma membrane damage (in 100% of treated hyphae). Further analysis of AFB1 production and gene expression related to secondary metabolism (laeA) and the mechanism of virulence (lipA and meT) of A. flavus in the presence of thyme EO indicated important physiological changes related to its anti-aflatoxigenic property. These results highlight the potent antifungal abilities of thyme EO in controlling A. flavus and AFB1 production, especially the abilities that operate by exerting changes at the molecular level and inducing significant apoptotic-like cell death.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aspergillus flavus / Aceites Volátiles / Thymus (Planta) / Antifúngicos Idioma: En Revista: Food Chem Año: 2020 Tipo del documento: Article País de afiliación: México

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aspergillus flavus / Aceites Volátiles / Thymus (Planta) / Antifúngicos Idioma: En Revista: Food Chem Año: 2020 Tipo del documento: Article País de afiliación: México
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