Absolute quantitation of high abundant Fc-glycopeptides from human serum IgG-1.
Anal Chim Acta
; 1102: 130-139, 2020 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-32043992
ABSTRACT
Absolute quantitation of IgG-1 Fc-glycosylation, which is crucial for the clinical practice of glyco-biomarkers and quality control of biopharmaceuticals, has been hindered by the lack of glycopeptide standards. In this study, eleven high abundant IgG-1 Fc-glycopeptides with definite peptide sequences and glycoforms were purified from commercial IgG protein by using two-dimensional hydrophilic interaction liquid chromatographic system. Based on the acquired glycopeptide standards, an absolute quantitation strategy was developed to determine the concentrations of 11 target IgG-1 glycopeptides from pooled human sera. A wide range of Fc-glycopeptide concentrations from 0.60 to 17.61 nmol mL-1 was achieved with excellent accuracy and reproducibility from pooled human sera IgG-1. Compared to conventional relative quantitation, this strategy provides more accurate distribution profiles of 11 high abundant Fc-glycopeptides and degree of glycosylation from pooled human sera IgG-1.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Inmunoglobulina G
/
Fragmentos Fc de Inmunoglobulinas
/
Glicopéptidos
Tipo de estudio:
Guideline
Límite:
Humans
Idioma:
En
Revista:
Anal Chim Acta
Año:
2020
Tipo del documento:
Article