Altered gene expression in CHO cells following polyamine starvation.
Biotechnol Lett
; 42(6): 927-936, 2020 Jun.
Article
en En
| MEDLINE
| ID: mdl-32078082
ABSTRACT
AIM:
To investigate the impact of polyamine deprivation on the transcriptome of CHO cellsRESULTS:
Polyamines play a central but poorly-understood role in cell proliferation. Most studies to date have utilised chemical inhibitors to probe polyamine function. Here we exploit the fact that CHO cells grown in serum-free medium have an absolute requirement for putrescine supplementation due to their deficiency in activity of the enzyme arginase. A gene expression microarray (Affymetrix) analysis of CHO-K1 cells starved of polyamines for 3 days showed that cessation of growth, associated with increased G1/S transition and inhibition of M/G1 transition was accompanied by increased mRNA levels of mitotic complex checkpoint genes (Mad2l1, Tkk, Bub1b) and in the transition of G1- to S-phase (such as Skp2 and Tfdp1). mRNAs associated with DNA homologous recombination and repair (including Fanconi's anaemia-related genes) and with RNA splicing were consistently increased. Alterations in mRNA levels for genes related to protein processing in the ER, to ER stress, and to p53-related and apoptosis pathways were also observed. mRNAs showing highest levels of fold-change included several which code for membrane-localised proteins and receptors (Thbs1, Tfrc1, Ackr3, Extl1).CONCLUSIONS:
Growth-arrest induced by polyamine deprivation was associated with significant alterations in levels of mRNAs associated with cell cycle progression, DNA repair, RNA splicing, ER trafficking and membrane signalling as well as p53 and apoptosis-related pathways.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Putrescina
/
Regulación de la Expresión Génica
/
Medios de Cultivo
/
Transcriptoma
Límite:
Animals
Idioma:
En
Revista:
Biotechnol Lett
Año:
2020
Tipo del documento:
Article
País de afiliación:
Irlanda