Effects of the DAG analogue 1,2-dioctanoyl-sn-glycerol (DiC8) on nicotine- and clothianidin-evoked currents through α-bungarotoxin-insensitive nicotinic acetylcholine receptors expressed on cockroach neurosecretory cells.
Neurotoxicology
; 78: 143-151, 2020 05.
Article
en En
| MEDLINE
| ID: mdl-32169464
ABSTRACT
We previously demonstrated that the cockroach α-bungarotoxin-sensitive nicotinic acetylcholine receptors, nAChR1 and nAChR2 subtypes, are differently sensitive to intracellular calcium pathways. Here, using whole cell patch-clamp recordings, we studied the effects of the diacylglycerol (DAG) analogue 1,2-dioctanoyl-sn-glycerol (DiC8) on nicotine- and clothianidin-evoked currents under an α-bungarotoxin treatment. Our results demonstrated that DiC8 reduced nicotine and clothianidin evoked currents. 10⯵M DiC8 suppressed the increase in nicotine-induced currents which was brought about by application of 5â¯mM caffeine or 9â¯mM Ca2+, whereas DiC8 did not affect the decrease in nicotine-induced currents induced by BAPTA. Similarly, bath application of caffeine or 9â¯mM Ca2+ did not change the clothianidin effects, and the amplitude of clothianidin-induced currents was not affected. However, co-application of both 10⯵M DiC8 with 9â¯mM Ca2+, caffeine or BAPTA reduced clothianidin current amplitudes. We conclude that nicotine and clothianidin differently modulate nAChR1 and nAChR2 subtypes under DiC8 treatment, and that nicotine activates nAChR1, whereas clothianidin activates both nAChR1 and nAChR2 subtypes.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tiazoles
/
Bungarotoxinas
/
Receptores Nicotínicos
/
Diglicéridos
/
Neonicotinoides
/
Guanidinas
/
Potenciales de la Membrana
/
Neuronas
/
Nicotina
Límite:
Animals
Idioma:
En
Revista:
Neurotoxicology
Año:
2020
Tipo del documento:
Article
País de afiliación:
Francia