Discovery of CC bond-forming and bond-breaking radical enzymes: enabling transformations for metabolic engineering.
Curr Opin Biotechnol
; 65: 94-101, 2020 10.
Article
en En
| MEDLINE
| ID: mdl-32171888
ABSTRACT
Radical enzymes catalyze some of the most chemically challenging CC bond-forming and bond-breaking reactions. Advances in DNA sequencing have accelerated the discovery of radical enzymes from microbes, including radical S-adenosylmethionine (rSAM) enzymes, glycyl radical enzymes (GREs), and diiron enzymes. These enzymes catalyze various reactions that yield products of industrial relevance (e.g. aromatics, hydrocarbons, and natural product derivatives), making their incorporation into engineered metabolic pathways enticing. Elucidating the mechanisms of radical enzymes that cleave and construct CC bonds will enable further enzyme discovery and engineering efforts.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Productos Biológicos
/
Ingeniería Metabólica
Idioma:
En
Revista:
Curr Opin Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos