Ta@Ag Porous Array with High Stability and Biocompatibility for SERS Sensing of Bacteria.

The reliable sensing of bacteria by surface-enhanced Raman scattering (SERS) technology necessitates a rational design of a substrate with high sensitivity, stability, and minimal invasion. Hence, a bimetallic Ta@Ag film with a porous array is developed by the magnetron sputtering technique and the structure could be controlled by a Ta dopant. A porous array connected by ligaments with compact granular nanoprotrusions is a fascinating substrate for SERS sensing. It makes steady SERS signals even in harsh chemical environments due to its high structural and chemical stability. The configuration of binary Ta@Ag has higher surface free energy than that of pure Ag, and the strong bactericidal activity of Ag is suppressed efficiently. Using E. coli as a model pathogen, the Ta@Ag porous film could maintain the long-term survival rate of E. coli up to 95% and a limit of SERS detection of E. coli down to 102 CFU/mL, which is measured by the standard colony-counting method. In sum, this work provides a promising strategy to fabricate a corrosion-resistant and biocompatible bimetallic Ta@Ag film with a porous array for the SERS sensing of microbial cells.