[Effect of fire-needle intervention on joint function, cartilage impairment and inflammatory response in knee osteoarthritis rats].
Zhen Ci Yan Jiu
; 45(3): 220-6, 2020 Mar 25.
Article
en Zh
| MEDLINE
| ID: mdl-32202714
ABSTRACT
OBJECTIVE:
To observe the effect of fire-needle stimulation of "Neixiyan"(EX-LE4) and "Dubi"(ST35) on changes of motor function, structure of cartilage degradation and inflammatory factors in knee osteoarthritis (KOA) rats, so as to explore its underlying mechanisms in improving KOA.METHODS:
Thirty-nine male SD rats were randomly divided into normal, model and fire-needle groups (nï¼13 in each group). The KOA model was established by injection of Monoiodoacetate (MIA, 1 mg) into the lumen of the right knee joint. On the 7th day after successful modeling, fire-needle was applied to EX-LE4 and ST35, twice a week for 3 weeks. The rats' behavioral reactions of gait (0 to 3 points) and claw pressure (0 to 3 points) were scored, and histopathological changes scored by assessing the impairment grade (0 to 6) and stage (0 to 4) of the articular cartilage after safranin O-fast green staining. The contents of serum Interleukin (IL)-1αï¼ Tumor necrosis factor α(TNF-α), IL-10, IL-37 and transforming growth factor ß(TGF-ß) were assayed by ELISA, and ultrastructural changes were observed under transmission electron microscope(TEM).RESULTS:
After modeling, the gait and claw-pressure scores were significantly increased in the model group in comparison with the normal group (P<0.05), and the OA score and contents of serum IL-1α and TNF-α were also appa-rently increased in the model group relevant to the normal group (P<0.05ï¼P<0.01), while the levels of serum IL-10, IL-37 and TGF-ß remarkably decreased in the model group in contrast to the normal group (P<0.01). After the intervention, the increase of gait and claw-pressure and OA scores, as well as serum IL-1α and TNF-α contents, and the decrease of serum IL-10, IL-37 and TGF-ß levels were all reversed in the fire needle group (P<0.05, P<0.01). Outcomes of TEM showed more and larger lipid droplets, swollen mitochondria with some vacuoles, and expanded, broken or dissolved rough endoplasmic reticulum in the model group, which was milder in the fire-needle group.CONCLUSION:
Fire-needle can improve motor function and relieve impairment of articular cartilage of KOA rats, which may be related to its effects in reducing inflammatory factors and in increasing anti-inflammatory factor levels.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cartílago Articular
/
Osteoartritis de la Rodilla
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Zhen Ci Yan Jiu
Asunto de la revista:
TERAPIAS COMPLEMENTARES
Año:
2020
Tipo del documento:
Article