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Fast pH-mediated changes of the viscosity of protein solutions studied with a voltage-modulated quartz crystal microbalance.
Gödde, Alexander; Leppin, Christian; Meyer, Frederick S; Langhoff, Arne; Hartl, Josef; Garidel, Patrick; Johannsmann, Diethelm.
Afiliación
  • Gödde A; Institute of Physical Chemistry, Clausthal University of Technology, Arnold-Sommerfeld-Str. 4, D-38678 Clausthal-Zellerfeld, Germany.
  • Leppin C; Institute of Physical Chemistry, Clausthal University of Technology, Arnold-Sommerfeld-Str. 4, D-38678 Clausthal-Zellerfeld, Germany.
  • Meyer FS; Institute of Physical Chemistry, Clausthal University of Technology, Arnold-Sommerfeld-Str. 4, D-38678 Clausthal-Zellerfeld, Germany.
  • Langhoff A; Institute of Physical Chemistry, Clausthal University of Technology, Arnold-Sommerfeld-Str. 4, D-38678 Clausthal-Zellerfeld, Germany.
  • Hartl J; Boehringer Ingelheim Pharma GmbH & Co. KG, Innovation Unit, PDB, Birkendorfer Str. 65, D-88397 Biberach/Riss, Germany.
  • Garidel P; Boehringer Ingelheim Pharma GmbH & Co. KG, Innovation Unit, PDB, Birkendorfer Str. 65, D-88397 Biberach/Riss, Germany.
  • Johannsmann D; Institute of Physical Chemistry, Clausthal University of Technology, Arnold-Sommerfeld-Str. 4, D-38678 Clausthal-Zellerfeld, Germany.
Biointerphases ; 15(2): 021004, 2020 03 24.
Article en En | MEDLINE | ID: mdl-32208731
An electrochemical quartz crystal microbalance is described, which achieves a time resolution down to 100 µs. Accumulation and averaging over a few hours bring the noise down to about 30 mHz. The application examples are pH-driven viscosity changes in albumin solutions. The pH was switched with the electrode potential. The characteristic response time is in the millisecond range. The focus is on experimental aspects as well as advantages and limitations of the technique.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Albúmina Sérica Bovina / Electricidad / Tecnicas de Microbalanza del Cristal de Cuarzo Límite: Animals Idioma: En Revista: Biointerphases Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2020 Tipo del documento: Article País de afiliación: Alemania
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Albúmina Sérica Bovina / Electricidad / Tecnicas de Microbalanza del Cristal de Cuarzo Límite: Animals Idioma: En Revista: Biointerphases Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2020 Tipo del documento: Article País de afiliación: Alemania