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The effect of a nanofiber-hydrogel composite on neural tissue repair and regeneration in the contused spinal cord.
Li, Xiaowei; Zhang, Chi; Haggerty, Agnes E; Yan, Jerry; Lan, Michael; Seu, Michelle; Yang, Mingyu; Marlow, Megan M; Maldonado-Lasunción, Inés; Cho, Brian; Zhou, Zhengbing; Chen, Long; Martin, Russell; Nitobe, Yohshiro; Yamane, Kentaro; You, Hua; Reddy, Sashank; Quan, Da-Ping; Oudega, Martin; Mao, Hai-Quan.
Afiliación
  • Li X; Translational Tissue Engineering Center, Baltimore, MD 21205, USA; Department of Materials Science & Engineering, Baltimore, MD 21205, USA; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Zhang C; Translational Tissue Engineering Center, Baltimore, MD 21205, USA; Department of Materials Science & Engineering, Baltimore, MD 21205, USA; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21205, USA; School of Chemistry, Sun Yat-Sen University, Guangzhou, Guangdong 51027
  • Haggerty AE; The Miami Project to Cure Paralysis, University of Miami, Miami, FL 33136, USA.
  • Yan J; Translational Tissue Engineering Center, Baltimore, MD 21205, USA; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Lan M; Translational Tissue Engineering Center, Baltimore, MD 21205, USA; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Seu M; Translational Tissue Engineering Center, Baltimore, MD 21205, USA; Department of Plastic and Reconstructive Surgery, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
  • Yang M; Translational Tissue Engineering Center, Baltimore, MD 21205, USA; Department of Materials Science & Engineering, Baltimore, MD 21205, USA; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Marlow MM; The Miami Project to Cure Paralysis, University of Miami, Miami, FL 33136, USA.
  • Maldonado-Lasunción I; The Miami Project to Cure Paralysis, University of Miami, Miami, FL 33136, USA; Department of Regeneration of Sensorimotor Systems, Netherlands Institute for Neuroscience, Institute of the Royal Netherlands Academy of Arts and Sciences, Amsterdam, Netherlands; Shirley Ryan AbilityLab, Chicago, IL 60
  • Cho B; Translational Tissue Engineering Center, Baltimore, MD 21205, USA; Department of Plastic and Reconstructive Surgery, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
  • Zhou Z; Translational Tissue Engineering Center, Baltimore, MD 21205, USA; Department of Materials Science & Engineering, Baltimore, MD 21205, USA; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Chen L; Translational Tissue Engineering Center, Baltimore, MD 21205, USA; Department of Materials Science & Engineering, Baltimore, MD 21205, USA; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Martin R; Translational Tissue Engineering Center, Baltimore, MD 21205, USA; Department of Materials Science & Engineering, Baltimore, MD 21205, USA; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Nitobe Y; The Miami Project to Cure Paralysis, University of Miami, Miami, FL 33136, USA; Department of Orthopedic Surgery, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori, 036-8562, Japan.
  • Yamane K; The Miami Project to Cure Paralysis, University of Miami, Miami, FL 33136, USA; Department of Orthopedic Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science, Kitaku, Okayama, 700-8558, Japan.
  • You H; Affiliated Cancer Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510095, PR China.
  • Reddy S; Department of Plastic and Reconstructive Surgery, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
  • Quan DP; School of Chemistry, Sun Yat-Sen University, Guangzhou, Guangdong 510275, PR China. Electronic address: cesqdp@mail.sysu.ed.cn.
  • Oudega M; Shirley Ryan AbilityLab, Chicago, IL 60611, USA; Department of Physical Therapy and Human Movements Sciences, Chicago, IL 60611, USA; Department of Physiology Northwestern University, Chicago, IL 60611, USA; Affiliated Cancer Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510095, PR Ch
  • Mao HQ; Translational Tissue Engineering Center, Baltimore, MD 21205, USA; Department of Materials Science & Engineering, Baltimore, MD 21205, USA; Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Biomedical Engineering, Johns Hopkins University, Baltimo
Biomaterials ; 245: 119978, 2020 07.
Article en En | MEDLINE | ID: mdl-32217415
ABSTRACT
An injury to the spinal cord causes long-lasting loss of nervous tissue because endogenous nervous tissue repair and regeneration at the site of injury is limited. We engineered an injectable nanofiber-hydrogel composite (NHC) with interfacial bonding to provide mechanical strength and porosity and examined its effect on repair and neural tissue regeneration in an adult rat model of spinal cord contusion. At 28 days after treatment with NHC, the width of the contused spinal cord segment was 2-fold larger than in controls. With NHC treatment, tissue in the injury had a 2-fold higher M2/M1 macrophage ratio, 5-fold higher blood vessel density, 2.6-fold higher immature neuron presence, 2.4-fold higher axon density, and a similar glial scar presence compared with controls. Spared nervous tissue volume in the contused segment and hind limb function was similar between groups. Our findings indicated that NHC provided mechanical support to the contused spinal cord and supported pro-regenerative macrophage polarization, angiogenesis, axon growth, and neurogenesis in the injured tissue without any exogenous factors or cells. These results motivate further optimization of the NHC and delivery protocol to fully translate the potential of the unique properties of the NHC for treating spinal cord injury.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Traumatismos de la Médula Espinal / Nanofibras Tipo de estudio: Guideline / Prognostic_studies Límite: Animals Idioma: En Revista: Biomaterials Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Traumatismos de la Médula Espinal / Nanofibras Tipo de estudio: Guideline / Prognostic_studies Límite: Animals Idioma: En Revista: Biomaterials Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos