Proteomic analysis of murine macrophages mitochondria and lysosomes reveal Cathepsin D as a potential broad-spectrum antimicrobial protein.
J Proteomics
; 223: 103821, 2020 07 15.
Article
en En
| MEDLINE
| ID: mdl-32417293
ABSTRACT
Bacterial resistance to antibiotics has become increasingly widespread, posing a serious threat to human life and health. Macrophages in the host's natural immune system can directly destroy most of bacteria. Therefore, exploring the function of macrophages' mitochondria and lysosomes in killing bacteria might help us overcome the problem of bacterial resistance. We used mass spectrometry to analyze the dynamic expression landscape of mitochondrial and lysosomal proteins in macrophages upon infection with Listeria monocytogenes, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. We discovered that Cathepsin D (Ctsd) is up-regulated at the protein level during infection by all five bacteria. Ctsd inhibitor and knockout experiments confirmed that Ctsd is a potential broad-spectrum antibacterial protein. Ctsd should be investigated further as a potential drug target for new antibacterial treatments.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Catepsina D
/
Proteómica
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Proteomics
Asunto de la revista:
BIOQUIMICA
Año:
2020
Tipo del documento:
Article
País de afiliación:
China