Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain.
Biotechnol Rep (Amst)
; 26: e00457, 2020 Jun.
Article
en En
| MEDLINE
| ID: mdl-32420050
ABSTRACT
The present work studied the optimization of aeration rate, agitation rate and oxygen transfer and the use of various batch fermentation strategies for xylanase production from a recombinant Aspergillus nidulans strain in a 3â¯L stirred tank reactor. Maximum xylanase production of 1250 U/mL with productivity of 313 U/mL/day was obtained under an aeration rate of 2 vvm and an agitation rate of 400â¯rpm using batch fermentation. The optimum volumetric oxygen transfer coefficient (kLa) for efficient xylanase production was found to be 38.6â¯h-1. Fed batch mode and repeated batch fermentation was also performed with kLa was 38.6â¯h-1. Xylanase enzyme productivity increased to 327 with fed batch fermentation and 373 U/mL/day with repeated batch fermentation. Also, maximum xylanase activity increased to 1410 U/mL with fed batch fermentation and 1572 U/mL with repeated batch fermentation.
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01-internacional
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MEDLINE
Idioma:
En
Revista:
Biotechnol Rep (Amst)
Año:
2020
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Article