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Exogenous Expression of WNT7A in Leukemia-Derived Cell Lines Induces Resistance to Chemotherapeutic Agents.
Alvarez-Zavala, Monserrat; Barreto-Vargas, Christian; Torres-Reyes, Luis A; De la Peña-Castro, Roberto F; Aguilar-Lemarroy, Adriana; Jave-Suarez, Luis F.
Afiliación
  • Alvarez-Zavala M; Instituto de Investigacion en Inmunodeficiencias y VIH, Departamento de Clínicas Medicas, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
  • Barreto-Vargas C; Division de Inmunologia, Centro de Investigacion Biomedica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, Mexico.
  • Torres-Reyes LA; Departamento de Biologia Molecular y Genomica, CUCS, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
  • De la Peña-Castro RF; Division de Inmunologia, Centro de Investigacion Biomedica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, Mexico.
  • Aguilar-Lemarroy A; Division de Inmunologia, Centro de Investigacion Biomedica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, Mexico.
  • Jave-Suarez LF; Division de Inmunologia, Centro de Investigacion Biomedica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, Mexico.
Anticancer Agents Med Chem ; 20(12): 1504-1514, 2020.
Article en En | MEDLINE | ID: mdl-32436833
BACKGROUND: Dysregulations of the WNT pathway are implicated in the malignant transformation of different types of neoplasia. WNT7A is expressed in normal peripheral lymphocytes, but is decreased in the tumoral counterpart. Furthermore, the treatment of leukemic cells with recombinant WNT7A decreases proliferation, suggesting its possible use as a therapeutic biomolecule. This study aimed to evaluate the concomitant action of WNT7A and different chemotherapeutic agents over proliferation and cell death of leukemia/ lymphoma derived cell lines. METHODS: Ectopic expression of WNT7A was induced in CEM and BJAB cell lines by using a lentiviral system. RNA expression was analyzed by microarrays and qPCR, and protein expression was determined by Western Blot. Cell proliferation was measured by cell counting, metabolic activity by WST-1 assay, cell death and DNA content by flow cytometry. RESULTS: WNT7A ectopic expression was shown to decrease cell proliferation, but the apoptosis rate of leukemic cells was not altered. Moreover, these cells acquired resistance to doxorubicin, vincristine and MG-132. Cell cycle analysis reveals a decrease in G1 and an increase in S and G2 phases with a further upregulation of senescence- associated genes. Microarray analysis reveals that most gene expression changes were related to cancer and metabolic associated pathways. All those changes appear to be independent of the WNT canonical pathway regulation. CONCLUSION: WNT7A negatively regulates cell proliferation in leukemic cell lines and promotes resistance to chemotherapeutic agents by inducing a senescence-like phenotype independently of the WNT canonical pathway.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 6_ODS3_enfermedades_notrasmisibles Problema de salud: 6_leukemia Asunto principal: Vincristina / Doxorrubicina / Resistencia a Antineoplásicos / Proteínas Wnt / Leupeptinas / Antineoplásicos Límite: Humans Idioma: En Revista: Anticancer Agents Med Chem Asunto de la revista: ANTINEOPLASICOS / QUIMICA Año: 2020 Tipo del documento: Article País de afiliación: México

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 6_ODS3_enfermedades_notrasmisibles Problema de salud: 6_leukemia Asunto principal: Vincristina / Doxorrubicina / Resistencia a Antineoplásicos / Proteínas Wnt / Leupeptinas / Antineoplásicos Límite: Humans Idioma: En Revista: Anticancer Agents Med Chem Asunto de la revista: ANTINEOPLASICOS / QUIMICA Año: 2020 Tipo del documento: Article País de afiliación: México
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