A Kinase-Phosphatase-Transcription Factor Module Regulates Adventitious Root Emergence in Arabidopsis Root-Hypocotyl Junctions.
Mol Plant
; 13(8): 1162-1177, 2020 08 03.
Article
en En
| MEDLINE
| ID: mdl-32534220
ABSTRACT
Adventitious roots form from non-root tissues as part of normal development or in response to stress or wounding. The root primordia form in the source tissue, and during emergence the adventitious roots penetrate the inner cell layers and the epidermis; however, the mechanisms underlying this emergence remain largely unexplored. Here, we report that a regulatory module composed of the AP2/ERF transcription factor ABSCISIC ACID INSENSITIVE 4 (ABI4), the MAP kinases MPK3 and MPK6, and the phosphatase PP2C12 plays an important role in the emergence of junction adventitious roots (J-ARs) from the root-hypocotyl junctions in Arabidopsis thaliana. ABI4 negatively regulates J-AR emergence, preventing the accumulation of reactive oxygen species and death of epidermal cells, which would otherwise facilitate J-AR emergence. Phosphorylation by MPK3/MPK6 activates ABI4 and dephosphorylation by PP2C12 inactivates ABI4. MPK3/MPK6 also directly phosphorylate and inactivate PP2C12 during J-AR emergence. We propose that this "double-check" mechanism increases the robustness of MAP kinase signaling and finely regulates the local programmed cell death required for J-AR emergence.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
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Arabidopsis
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Raíces de Plantas
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Hipocótilo
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Quinasas de Proteína Quinasa Activadas por Mitógenos
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Proteínas Quinasas Activadas por Mitógenos
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Proteínas de Arabidopsis
Idioma:
En
Revista:
Mol Plant
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BOTANICA
Año:
2020
Tipo del documento:
Article
País de afiliación:
China