Mass spectrometry of urinary ß2 microglobulin oligomer in patients with proteinuria.
Ther Apher Dial
; 25(2): 197-203, 2021 Apr.
Article
en En
| MEDLINE
| ID: mdl-32535961
ABSTRACT
Urinary ß2 microglobulin (ß2-m) is a marker of renal tubule dysfunction; however, ß2-m might become degraded under acidic conditions. To confirm the degradation and consequent deactivation of ß2-m under acidic conditions, we used matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) to detect the levels and forms of ß2-m in the urine samples of patients with high proteinuria (n = 21) and healthy subjects (n = 6). ß2-m was purified in crude form using immunoprecipitation. A signal of 11.74 kDa, corresponding to the molecular weight of ß2-m, was detected in all samples. In addition, several high-molecular-weight proteins were detected in a patient as integrals of the intensity at 11.74 kDa. These results indicate that posttranslational modifications of ß2-m might be involved in the pathological process of proteinuria. Therefore, MS can be used for monitoring proteinuria and predicting the risk of progression.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteinuria
/
Microglobulina beta-2
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Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
Tipo de estudio:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Límite:
Aged
/
Aged80
/
Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Ther Apher Dial
Asunto de la revista:
HEMATOLOGIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Japón