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Comparing serum protein levels can aid in differentiating HPV-negative and -positive oropharyngeal squamous cell carcinoma patients.
Dickinson, Amy; Saraswat, Mayank; Syrjänen, Stina; Tohmola, Tiialotta; Silén, Robert; Randén-Brady, Reija; Carpén, Timo; Hagström, Jaana; Haglund, Caj; Mattila, Petri; Mäkitie, Antti; Joenväärä, Sakari; Silén, Suvi.
Afiliación
  • Dickinson A; Department of Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
  • Saraswat M; Research Program in Systems Oncology, Faculty of Medicine, University of Helsinki, Helsinki, Finland.
  • Syrjänen S; Transplantation Laboratory, Haartman Institute, University of Helsinki, Helsinki, Finland.
  • Tohmola T; HUSLAB, Helsinki University Hospital, Helsinki, Finland.
  • Silén R; Department of Oral Pathology and Oral Radiology, University of Turku, Turku, Finland.
  • Randén-Brady R; Department of Pathology, Turku University Hospital, Turku, Finland.
  • Carpén T; Transplantation Laboratory, Haartman Institute, University of Helsinki, Helsinki, Finland.
  • Hagström J; Transplantation Laboratory, Haartman Institute, University of Helsinki, Helsinki, Finland.
  • Haglund C; Department of Pathology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
  • Mattila P; Department of Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
  • Mäkitie A; Department of Pathology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
  • Joenväärä S; Department of Oral Pathology and Oral Radiology, University of Turku, Turku, Finland.
  • Silén S; Department of Pathology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
PLoS One ; 15(6): e0233974, 2020.
Article en En | MEDLINE | ID: mdl-32542012
BACKGROUND: The surrogate immunohistochemical marker, p16INK4a, is used in clinical practice to determine the high-risk human papillomavirus (HPV) status of oropharyngeal squamous cell carcinomas (OPSCC). With a specificity of 83%, this will misclassify some patients compared with direct HPV testing. Patients who are p16INK4a-positive but HPV DNA-negative, or RNA-negative, may be unsuitable for treatment de-escalation aimed at reducing treatment-related side effects. We aimed to identify cost-effective serum markers to improve decision making for patients at risk of misclassification by p16INK4a alone. METHODS: Serum proteins from pre-treatment samples of 36 patients with OPSCC were identified and quantified using label-free mass spectrometry-based proteomics. HPV-status was determined using p16INK4a/HPV DNA and E6/E7 mRNA. Serum protein expressions were compared between groups of patients according to HPV status, using the unpaired t-test with a Benjamini-Hochberg correction. ROC curves (AUC) were calculated with SPSS (v25). RESULTS: Of 174 serum proteins identified, complement component C7 (C7), apolipoprotein F (ApoF) and galectin-3-Binding Protein (LGALS3BP) significantly differed between HPV-positive and -negative tumors (AUC ranging from 0.84-0.87). ApoF levels were more than twice as high in the E6/E7 mRNA HPV-positive group than HPV-negative. CONCLUSIONS: Serum C7, ApoF and LGALS3BP levels discriminate between HPV-positive and HPV-negative OPSCC. Further studies are needed to validate these host immunity-related proteins as markers for HPV-associated OPSCC.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 2_ODS3 Problema de salud: 2_enfermedades_transmissibles Asunto principal: Papillomaviridae / Apolipoproteínas / Complemento C7 / Neoplasias Orofaríngeas / Biomarcadores de Tumor / Infecciones por Papillomavirus / Carcinoma de Células Escamosas de Cabeza y Cuello / Antígenos de Neoplasias Tipo de estudio: Prognostic_studies Límite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2020 Tipo del documento: Article País de afiliación: Finlandia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 2_ODS3 Problema de salud: 2_enfermedades_transmissibles Asunto principal: Papillomaviridae / Apolipoproteínas / Complemento C7 / Neoplasias Orofaríngeas / Biomarcadores de Tumor / Infecciones por Papillomavirus / Carcinoma de Células Escamosas de Cabeza y Cuello / Antígenos de Neoplasias Tipo de estudio: Prognostic_studies Límite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2020 Tipo del documento: Article País de afiliación: Finlandia
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