Metabolic changes in mouse sperm during capacitation.
Biol Reprod
; 103(4): 791-801, 2020 10 05.
Article
en En
| MEDLINE
| ID: mdl-32614044
ABSTRACT
Mammalian sperm are stored in the epididymis in a dormant state. Upon ejaculation, they must immediately start producing sufficient energy to maintain motility and support capacitation. While this increased energy demand during capacitation is well established, it remains unclear how mouse sperm modify their metabolism to meet this need. We now show that capacitating mouse sperm enhance glucose uptake, identifying glucose uptake as a functional marker of capacitation. Using an extracellular flux analyzer, we show that glycolysis and oxidative phosphorylation increase during capacitation. Furthermore, this increase in oxidative phosphorylation is dependent on glycolysis, providing experimental evidence for a link between glycolysis and oxidative phosphorylation in mouse sperm.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Capacitación Espermática
/
Espermatozoides
/
Metabolismo Energético
Límite:
Animals
Idioma:
En
Revista:
Biol Reprod
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos