Screening and semi-quantitative determination of pentacyclic triterpenoids in plants by liquid chromatography-tandem mass spectrometry in precursor ion scan mode.
Phytochem Anal
; 32(3): 252-261, 2021 May.
Article
en En
| MEDLINE
| ID: mdl-32638461
ABSTRACT
INTRODUCTION:
Pentacyclic triterpenoids (PCTs) are secondary plant metabolites. They are of exceptional interest as biologically active substances and raw materials for a wide range of medications. Thus, the development of a methodology for rapid screening of PCTs in plant biomass is an important task.OBJECTIVE:
The goal of this work was to develop an approach for simultaneous screening and semi-quantitative determination of PCTs in plant tissues by liquid chromatography-tandem mass spectrometry with a precursor ion scan (PrecIS). MATERIALS ANDMETHODS:
Pressurised liquid extraction (PLE) with methanol was used for the isolation of PCTs from plant biomass. Screening and semi-quantitative determination of PCTs in the obtained extracts were carried out by reversed phase high-performance liquid chromatography-tandem mass spectrometry in a PrecIS mode.RESULTS:
The product ion at m/z 95 with collision energy of 40 V was used as a diagnostic ion to identify PCTs by the PrecIS mode. In plant materials, 26 PCTs and their derivatives, such as PCTs esters and glycosides, were detected and identified. Calculation of the relative response factor for nine available PCTs showed that using a betulin calibration curve allows us to estimate the semi-quantitative content of PCTs and their derivatives in plant PLE extracts.CONCLUSION:
The developed approach can be applied for simultaneous untargeted screening and semi-quantitative determination of PCTs and their derivatives in various plants at sub-parts per million levels.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Triterpenos
/
Espectrometría de Masas en Tándem
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Idioma:
En
Revista:
Phytochem Anal
Asunto de la revista:
BOTANICA
/
QUIMICA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Rusia