Efficient sequential synthesis of lacto-N-triose II and lacto-N-neotetraose by a novel ß-N-acetylhexosaminidase from Tyzzerella nexilis.
Food Chem
; 332: 127438, 2020 Dec 01.
Article
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| MEDLINE
| ID: mdl-32645671
ABSTRACT
ß-N-acetylhexosaminidases have attracted much attention in recent years due to their potential application in oligosaccharide production, in particular lacto-N-triose II (LNT2) and lacto-N-neotetraose (LNnT) synthesis, which can be further used as backbone precursors for human milk oligosaccharides. A novel ß-N-acetylhexosaminidase gene from Tyzzerella nexilis (TnHex189) was heterologously expressed in Bacillus subtilis. The highest ß-N-acetylhexosaminidase activity of 14.5 U mL-1 was obtained in a 5-L fermentor by fed-batch fermentation for 27 h. TnHex189 was optimally active at pH 5.0 and 45 °C. It efficiently synthesized LNT2 with a conversion ratio of 57.2% (4.7 g L-1). The synthesized LNT2 was further converted to LNnT by a reported ß-galactosidase (BgaD-D) in 8 h, with a conversion ratio of 17.3% (6.1 g L-1). These unique synthesis activities may make this enzyme a good candidate for the food industry.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
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Trisacáridos
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Beta-N-Acetilhexosaminidasas
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Clostridiales
Idioma:
En
Revista:
Food Chem
Año:
2020
Tipo del documento:
Article
País de afiliación:
China