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Electroactive polypyrrole-molybdenum disulfide nanocomposite for ultrasensitive detection of berberine in rat plasma.
Wang, Jun-Sheng; Sakthivel, Rajalakshmi; Anbazhagan, Rajeshkumar; Krishnamoorthi, Rajakumari; Kubendhiran, Subbiramaniyan; Lai, Juin-Yih; Tsai, Hsieh-Chih; Chen, Shen-Ming.
Afiliación
  • Wang JS; Taiwan Instrument Research Institute, National Applied Research Laboratories, Hsinchu, Taiwan.
  • Sakthivel R; Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan.
  • Anbazhagan R; Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan; Advanced Membrane Materials Research Center, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan. Electro
  • Krishnamoorthi R; Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan; Advanced Membrane Materials Research Center, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan.
  • Kubendhiran S; Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
  • Lai JY; Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan; Advanced Membrane Materials Research Center, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan; R&D
  • Tsai HC; Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan; Advanced Membrane Materials Research Center, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan; R&D
  • Chen SM; Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan. Electronic address: smchen@ntut.edu.tw.
Anal Chim Acta ; 1125: 210-219, 2020 Aug 15.
Article en En | MEDLINE | ID: mdl-32674768
ABSTRACT
Electroactive polypyrrole-molybdenum disulfide (MoP) nanocomposites were synthesized and used for modifying screen-printed carbon electrodes (SPCEs) for ultrasensitive detection of berberine, an anticancer drug, in rat plasma. The electroactive nanocomposites were fabricated by exfoliating MoS2 followed by pyrrole polymerization. The effect of polypyrrole in the MoP nanocomposite was evaluated by varying the pyrrole concentration during polymerization, and the resulting nanocomposites prepared with pyrrole concentrations of 10, 20, 30 µL were named as MoP-1, MoP-2, and MoP-3, respectively. The electrochemical characterization of the three MoP nanocomposite sensors revealed that MoP-2/SPCE exhibited the highest electroactivity. The detection of berberine by the three MoP-coated SPCEs revealed that MoP-2/SPCE exhibited the highest activity against berberine due to a two-electron transfer mechanism on the MoP-2/SPCE electrode surface. The detection limit of berberine using the MoP-2/SPCE sensor was found to be about 0.05 µM, which is remarkably lower than the reported detection limits. The interference study proved the selectivity of the MoP-2/SPCE sensor toward berberine in the presence of other bioactive molecules and metal ions. The designed MoP-2/SPCE sensor retained 92% of its initial activity after 15 days of storage at room temperature, with RSD values of about 2.95% and 3.68% for the repeatability and reproducibility studies. Finally, the detection limit of berberine in a rat plasma sample determined using the MoP-2/SPCE sensor was found to be about 5 µM.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Pirroles / Berberina / Disulfuros / Nanocompuestos / Molibdeno Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Anal Chim Acta Año: 2020 Tipo del documento: Article País de afiliación: Taiwán
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Pirroles / Berberina / Disulfuros / Nanocompuestos / Molibdeno Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Anal Chim Acta Año: 2020 Tipo del documento: Article País de afiliación: Taiwán