DropSynth 2.0: high-fidelity multiplexed gene synthesis in emulsions.
Nucleic Acids Res
; 48(16): e95, 2020 09 18.
Article
en En
| MEDLINE
| ID: mdl-32692349
ABSTRACT
Multiplexed assays allow functional testing of large synthetic libraries of genetic elements, but are limited by the designability, length, fidelity and scale of the input DNA. Here, we improve DropSynth, a low-cost, multiplexed method that builds gene libraries by compartmentalizing and assembling microarray-derived oligonucleotides in vortexed emulsions. By optimizing enzyme choice, adding enzymatic error correction and increasing scale, we show that DropSynth can build thousands of gene-length fragments at >20% fidelity.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Oligonucleótidos
/
Biblioteca de Genes
/
Técnicas de Amplificación de Ácido Nucleico
/
Genes Sintéticos
Idioma:
En
Revista:
Nucleic Acids Res
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos