Grooved hydroxyapatite scaffold modulates mitochondria homeostasis and thus promotes osteogenesis in bone mesenchymal stromal cells.
Mol Med Rep
; 22(4): 2801-2809, 2020 10.
Article
en En
| MEDLINE
| ID: mdl-32700750
ABSTRACT
Hydroxyapatite scaffolds (HASs) are widely studied as suitable materials for bone replacement scaffolds due to their chemical similarities to organic materials. In our previous study, a novel HAS with a 2530µm groove structure (HASG) exhibited enhanced osteogenesis of bone mesenchymal stromal cells (BMSCs) compared with HAS, potentially by modulating the macrophageinduced immune microenvironment. However, the exact effects of different surface patterns on the physiological processes of attached cells is not known. The present study aimed to determine the effects of HASG on the osteogenesis and physiological processes in BMSCs. Cell counting kit8 assays and propidium iodide staining followed by flow cytometry were performed, and the results demonstrated that both in normal medium and differentiating medium, HASG promoted cell proliferation by decreasing the proportion of G1/G0 cells and decreased reactive oxygen species (ROS) accumulation in BMSCs compared with HAS. Detection markers of osteogenesis revealed that compared with HAS, HASG increased runtrelated transcription factor 2, osteocalcin and osteopontin protein levels and promoted osteogenesis, which was further confirmed by Alizarin Red S staining. Following JC1 staining, it was observed that HASG maintained the mitochondrial membrane potential, similar to that achieved by Nacetylcysteine pretreatment. In addition, compared with those of HAS, HASG decreased mitochondrial ROS levels, which potentially contributed to the promotion of osteogenesis. The results also demonstrated that HASG inhibited mitophagy induced by ROS accumulation and ATP synthesis compared with HAS. In conclusion, HASG decreased ROS accumulation and mitophagy and thus promoted osteogenesis of BMSCs, indicating that ROS modulation of HASG may serve a key role in osteogenesis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Osteogénesis
/
Durapatita
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Andamios del Tejido
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Células Madre Mesenquimatosas
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Mitocondrias
Límite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Año:
2020
Tipo del documento:
Article