Off the wall: The rhyme and reason of Neurospora crassa hyphal morphogenesis.
Cell Surf
; 5: 100020, 2019 Dec.
Article
en En
| MEDLINE
| ID: mdl-32743136
The fungal cell wall building processes are the ultimate determinants of hyphal shape. In Neurospora crassa the main cell wall components, ß-1,3-glucan and chitin, are synthesized by enzymes conveyed by specialized vesicles to the hyphal tip. These vesicles follow different secretory routes, which are delicately coordinated by cargo-specific Rab GTPases until their accumulation at the Spitzenkörper. From there, the exocyst mediates the docking of secretory vesicles to the plasma membrane, where they ultimately get fused. Although significant progress has been done on the cellular mechanisms that carry cell wall synthesizing enzymes from the endoplasmic reticulum to hyphal tips, a lot of information is still missing. Here, the current knowledge on N. crassa cell wall composition and biosynthesis is presented with an emphasis on the underlying molecular and cellular secretory processes.
BGT, ß-1,3-glucan transferases; CHS, chitin synthase; CLSM, confocal laser scanning microscopy; CWI, cell wall integrity; CWP, cell wall proteins; Cell wall; ER, endoplasmic reticulum; FRAP, fluorescence recovery after photobleaching; GEF, guanine nucleotide exchange factor; GFP, green fluorescent protein; GH, glycosyl hydrolases; GPI, glycosylphosphatidylinositol; GSC, ß-1,3-glucan synthase complex; MMD, myosin-like motor domain; MS, mass spectrometry; MT, microtubule; NEC, network of elongated cisternae; PM, plasma membrane; SPK, Spitzenkörper; Spitzenkörper; TIRFM, total internal reflection fluorescence microscopy; TM, transmembrane; Tip growth; Vesicles
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Cell Surf
Año:
2019
Tipo del documento:
Article
País de afiliación:
México