Barley beta-Glucan and Zymosan induce Dectin-1 and Toll-like receptor 2 co-localization and anti-leishmanial immune response in Leishmania donovani-infected BALB/c mice.
Scand J Immunol
; 92(6): e12952, 2020 Dec.
Article
en En
| MEDLINE
| ID: mdl-32748397
ABSTRACT
Toll-like receptors (TLRs), TLR2 in particular, are shown to recognize various glycans and glycolipid ligands resulting in various immune effector functions. As barley ß-glucan and zymosan are the glycans implicated in immunomodulation, we examined whether these ligands interact with Dectin-1, a lectin-type receptor for glycans, and TLR2 and induce immune responses that can be used against Leishmania infection in a susceptible host. The binding affinity of barley ß-glucan and zymosan with Dectin-1 and TLR2 was studied in silico. Barley ß-glucan- and zymosan-induced dectin-1 and TLR2 co-localization was studied by confocal microscopy and co-immunoprecipitation. These ligands-induced signalling and effector functions were assessed by Western blot analyses and various immunological assays. Finally, the anti-leishmanial potential of barley ß-glucan and zymosan was tested in Leishmania donovani -infected macrophages and in L. donovani-infected BALB/c mice. Both barley ß-glucan and zymosan interacted with TLR2 and dectin-1, but with a much stronger binding affinity for the latter, and therefore induced co-localization of these two receptors on BALB/c-derived macrophages. Both ligandsactivated MyD88- and Syk-mediated downstream pathways for heightened inflammatory responses in L. donovani-infected macrophages. These two ligands induced T cell-dependent host protection in L. donovani-infected BALB/c mice. These results establish a novel modus operandi of ß-glucans through dectin-1 and TLR2 and suggest an immuno-modulatory potential against infectious diseases.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Zimosan
/
Leishmania donovani
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Lectinas Tipo C
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Beta-Glucanos
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Receptor Toll-Like 2
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Leishmaniasis Visceral
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Macrófagos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Scand J Immunol
Año:
2020
Tipo del documento:
Article
País de afiliación:
India