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Characterization and origins of cell-free mitochondria in healthy murine and human blood.
Stephens, Olivia R; Grant, Dillon; Frimel, Matthew; Wanner, Nicholas; Yin, Mei; Willard, Belinda; Erzurum, Serpil C; Asosingh, Kewal.
Afiliación
  • Stephens OR; Department of Inflammation and Immunity, Cleveland Clinic, Cleveland, OH, United States.
  • Grant D; Department of Inflammation and Immunity, Cleveland Clinic, Cleveland, OH, United States.
  • Frimel M; Department of Inflammation and Immunity, Cleveland Clinic, Cleveland, OH, United States.
  • Wanner N; Department of Inflammation and Immunity, Cleveland Clinic, Cleveland, OH, United States.
  • Yin M; Imaging Core, Cleveland Clinic, Cleveland, OH, United States.
  • Willard B; Proteomics and Metabolomics Core, Cleveland Clinic, Cleveland, OH, United States.
  • Erzurum SC; Department of Inflammation and Immunity, Cleveland Clinic, Cleveland, OH, United States.
  • Asosingh K; Department of Inflammation and Immunity, Cleveland Clinic, Cleveland, OH, United States; Flow Cytometry Core Lerner Research Institute, Cleveland Clinic, Cleveland, OH, United States. Electronic address: asosink@ccf.org.
Mitochondrion ; 54: 102-112, 2020 09.
Article en En | MEDLINE | ID: mdl-32781153
ABSTRACT
Intact cell-free mitochondria have been reported in microparticles (MPs) in murine and human bodily fluids under disease conditions. However, cellular origins of circulating extracellular mitochondria have not been characterized. We hypothesize that intact, cell-free mitochondria from heterogeneous cellular sources are present in the circulation under physiological conditions. To test this, circulating MPs were analyzed using flow cytometry and proteomics. Murine and human platelet-depleted plasma showed a cluster of MPs positive for the mitochondrial probe MitoTracker. Transgenic mice expressing mitochondrial-GFP showed GFP positivity in plasma MPs. Murine and human mitochondria-containing MPs were positive for the platelet marker CD41 and the endothelial cell marker CD144, while hematopoietic CD45 labeling was low. Both murine and human circulating cell-free mitochondria maintained a transmembrane potential. Circulating mitochondria were able to enter rho-zero cells, and were visualized using immunoelectron microscopic imaging. Proteomics analysis identified mitochondria specific and extracellular vesicle associated proteins in sorted circulating cell-free human mitochondria. Together the data provide multiple lines of evidence that intact and functional mitochondria originating from several cell types are present in the blood circulation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasma / Proteómica / Micropartículas Derivadas de Células / Mitocondrias Límite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: Mitochondrion Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasma / Proteómica / Micropartículas Derivadas de Células / Mitocondrias Límite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: Mitochondrion Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos
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