Generation of Random luxCDABE Transcriptional Fusions in the Genome of Salmonella enterica.
Methods Mol Biol
; 2182: 141-151, 2021.
Article
en En
| MEDLINE
| ID: mdl-32894493
ABSTRACT
The luxCDABE operon of Photorhabdus luminescens can be used as a bioluminescent reporter to measure gene transcription nondestructively. Here we describe protocols to (1) generate random transcriptional fusions of the lux operon to genes of the Salmonella genome, (2) screen for specific fusions with constitutive expression, Salmonella pathogenicity island 1-related expression, or Salmonella pathogenicity island 2-related expression, and (3) determine the site of luxCDABE integration.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Proteínas Bacterianas
/
Transcripción Genética
/
Genoma Bacteriano
/
Salmonella enterica
/
Photorhabdus
Tipo de estudio:
Clinical_trials
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2021
Tipo del documento:
Article
País de afiliación:
España