Neural expression of sorting nexin 25 and its regulation of tyrosine receptor kinase B trafficking.
Brain Struct Funct
; 225(9): 2615-2642, 2020 Dec.
Article
en En
| MEDLINE
| ID: mdl-32955616
ABSTRACT
Sorting nexin 25 (SNX25) belongs to the sorting nexin superfamily, whose members are responsible for membrane attachment to organelles of the endocytic system. Recent reports point to critical roles for SNX25 as a negative regulator of transforming growth factor ß signaling, but the expression patterns of SNX25 in the central nervous system (CNS) remain almost uncharacterized. Here, we show widespread neuronal expression of SNX25 protein and Snx25 mRNA using immunohistochemistry and in situ hybridization. As an exception, SNX25 was present in the Bergmann glia of the cerebellum. SNX25 immunoreactivity was found in cholinergic and catecholaminergic neurons. Moreover, SNX25 colocalized with tropomyosin receptor kinase B (TrkB) in the neurons of the cortex and hippocampus. In vitro, SNX25 can interact with full-length TrkB, but not with its C-terminal-truncated isoform. Overexpression of SNX25 accelerated degradation of full-lengh TrkB, indicating that SNX25 promotes the trafficking of TrkB for lysosomal degradation. These findings suggest that SNX25 is a new actor in endocytic signaling, perhaps contributing to the regulation of BDNF-TrkB signaling in the CNS.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Encéfalo
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Proteínas Tirosina Quinasas
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Glicoproteínas de Membrana
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Nexinas de Clasificación
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Neuronas
Límite:
Animals
Idioma:
En
Revista:
Brain Struct Funct
Asunto de la revista:
CEREBRO
Año:
2020
Tipo del documento:
Article
País de afiliación:
Japón