The condensin holocomplex cycles dynamically between open and collapsed states.
Nat Struct Mol Biol
; 27(12): 1134-1141, 2020 12.
Article
en En
| MEDLINE
| ID: mdl-32989304
ABSTRACT
Structural maintenance of chromosome (SMC) protein complexes are the key organizers of the spatiotemporal structure of chromosomes. The condensin SMC complex has recently been shown to be a molecular motor that extrudes large loops of DNA, but the mechanism of this unique motor remains elusive. Using atomic force microscopy, we show that budding yeast condensin exhibits mainly open 'O' shapes and collapsed 'B' shapes, and it cycles dynamically between these two states over time, with ATP binding inducing the O to B transition. Condensin binds DNA via its globular domain and also via the hinge domain. We observe a single condensin complex at the stem of extruded DNA loops, where the neck size of the DNA loop correlates with the width of the condensin complex. The results are indicative of a type of scrunching model in which condensin extrudes DNA by a cyclic switching of its conformation between O and B shapes.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
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ADN de Hongos
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Proteínas Fúngicas
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Proteínas Cromosómicas no Histona
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Cromosomas Fúngicos
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Adenosina Trifosfato
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Adenosina Trifosfatasas
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Complejos Multiproteicos
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Proteínas de Unión al ADN
Idioma:
En
Revista:
Nat Struct Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2020
Tipo del documento:
Article
País de afiliación:
Países Bajos