Advancing d-amino acid-containing peptide discovery in the metazoan.
Biochim Biophys Acta Proteins Proteom
; 1869(1): 140553, 2021 01.
Article
en En
| MEDLINE
| ID: mdl-33002629
ABSTRACT
The discovery of enzyme-derived d-amino acid-containing peptides (DAACPs) that have physiological importance in the metazoan challenges previous assumptions about the homochirality of animal proteins while simultaneously revealing new analytical challenges in the structural and functional characterization of peptides. Most known DAACPs have been identified though laborious activity-guided purification studies or by homology to previously identified DAACPs. Peptide characterization experiments are increasingly dominated by high throughput mass spectrometry-based peptidomics, with stereochemistry rarely considered due to the technical challenges of identifying l/d isomerization. This review discusses the prevalence of enzyme-derived DAACPs among animals and the physiological consequences of peptide isomerization. Also highlighted are the analytical methods that have been applied for structural characterization/discovery of DAACPs, including results of several recent studies using non-targeted discovery methods for revealing novel DAACPs, strongly suggesting that more DAACPs remain to be uncovered.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligopéptidos
/
Péptidos Cíclicos
/
Péptidos Opioides
/
Conotoxinas
/
Péptidos Catiónicos Antimicrobianos
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta Proteins Proteom
Año:
2021
Tipo del documento:
Article
País de afiliación:
Estados Unidos