SELEX-based DNA Aptamer Selection: A Perspective from the Advancement of Separation Techniques.
Anal Sci
; 37(1): 17-26, 2021 Jan 10.
Article
en En
| MEDLINE
| ID: mdl-33132238
ABSTRACT
DNA aptamers, which are short, single-stranded DNA sequences that selectively bind to target substances (proteins, cells, small molecules, metal ions), can be acquired by means of the systematic evolution of ligands by exponential enrichment (SELEX) methodology. In the SELEX procedure, one of the keys for the effective acquisition of high-affinity and functional aptamer sequences is the separation stage to isolate target-bound DNA from unbound DNA in a randomized DNA library. In this review, various remarkable advancements in separation techniques for SELEX-based aptamer selection developed in this decade, are described and discussed, including CE-, microfluidic chip-, solid phase-, and FACS-based SELEX along with other methods.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
Técnica SELEX de Producción de Aptámeros
Tipo de estudio:
Clinical_trials
Límite:
Humans
Idioma:
En
Revista:
Anal Sci
Año:
2021
Tipo del documento:
Article
País de afiliación:
Japón