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Mitochondrial DNA lesions and copy number are strain dependent in endurance-trained mice.
Vellers, Heather L; Massett, Michael P; Avila, Josh J; Kim, Seung Kyum; Marzec, Jacqui M; Santos, Janine H; Lightfoot, J Timothy; Kleeberger, Steven R.
Afiliación
  • Vellers HL; Department of Kinesiology and Sport Management, Texas Tech University, Lubbock, TX, USA.
  • Massett MP; Department of Kinesiology and Sport Management, Texas Tech University, Lubbock, TX, USA.
  • Avila JJ; Department of Health and Kinesiology, Texas A&M University College Station, College Station, TX, USA.
  • Kim SK; Division of Research, Texas A&M University College Station, College Station, TX, USA.
  • Marzec JM; Department of Sports Science, Seoul National University of Science and Technology, Seoul, Republic of Korea.
  • Santos JH; National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC, USA.
  • Lightfoot JT; National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC, USA.
  • Kleeberger SR; Department of Health and Kinesiology, Texas A&M University College Station, College Station, TX, USA.
Physiol Rep ; 8(21): e14605, 2020 11.
Article en En | MEDLINE | ID: mdl-33190396
ABSTRACT
In this pilot work, we selected two inbred strains that respond well to endurance training (ET) (FVB/NJ, and SJL/J strains), and two strains that respond poorly (BALB/cByJ and NZW/LacJ), to determine the effect of a standardized ET treadmill program on mitochondrial and nuclear DNA (nucDNA) integrity, and mitochondrial DNA (mtDNA) copy number. DNA was isolated from plantaris muscles (n = 37) and a gene-specific quantitative PCR-based assay was used to measure DNA lesions and mtDNA copy number. Mean mtDNA lesions were not different within strains in the sedentary or exercise-trained states. However, mtDNA lesions were significantly higher in trained low-responding NZW/LacJ mice (0.24 ± 0.06 mtDNA lesions/10 Kb) compared to high-responding strains (mtDNA lesions/10 Kb FVB/NJ = 0.11 ± 0.01, p = .049; SJL/J = 0.04 ± 0.02; p = .003). ET did not alter mean mtDNA copy numbers for any strain, although both sedentary and trained FVB/NJ mice had significantly higher mtDNA copies (99,890 ± 4,884 mtDNA copies) compared to low-responding strains (mtDNA copies BALB/cByJ = 69,744 ± 4,675; NZW/LacJ = 65,687 ± 5,180; p < .001). ET did not change nucDNA lesions for any strain, however, SJL/J had the lowest mean nucDNA lesions (3.5 ± 0.14 nucDNA lesions/6.5 Kb) compared to all other strains (nucDNA lesions/6.5 Kb FVB/NJ = 4.4 ± 0.11; BALB/cByJ = 4.7 ± 0.09; NZW/LacJ = 4.4 ± 0.11; p < .0001). Our results demonstrate strain differences in plantaris muscle mtDNA lesions in ET mice and, independent of condition, differences in mean mtDNA copy and nucDNA lesions between strains.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Condicionamiento Físico Animal / Daño del ADN / ADN Mitocondrial / Variaciones en el Número de Copia de ADN / Mitocondrias Límite: Animals Idioma: En Revista: Physiol Rep Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Condicionamiento Físico Animal / Daño del ADN / ADN Mitocondrial / Variaciones en el Número de Copia de ADN / Mitocondrias Límite: Animals Idioma: En Revista: Physiol Rep Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos
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