Intravital 2-Photon Microscopy of Diverse Cell Types in the Murine Tibia.
Methods Mol Biol
; 2236: 189-201, 2021.
Article
en En
| MEDLINE
| ID: mdl-33237549
ABSTRACT
Intravital imaging allows the visualization of fluorescently labeled structures like cells, blood flow, and pathogens in a living organism. Nowadays, numerous methods for imaging in several organs are available. In this chapter, we present a method for intravital 2-photon microscopy of the murine tibial bone marrow. It enables the observation of hematopoietic cells including cells of the innate and adaptive immune system under physiological conditions. Motility analyses within this complex environment led to insights into their migratory potential as well as their interactions with other cells or blood vessels.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tibia
/
Microscopía de Fluorescencia por Excitación Multifotónica
/
Microscopía Intravital
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2021
Tipo del documento:
Article
País de afiliación:
Alemania