How Have Leukocyte In Vitro Chemotaxis Assays Shaped Our Ideas about Macrophage Migration?
Biology (Basel)
; 9(12)2020 Dec 02.
Article
en En
| MEDLINE
| ID: mdl-33276594
ABSTRACT
Macrophage chemotaxis is crucial during both onset and resolution of inflammation and unique among all leukocytes. Macrophages are able to switch between amoeboid and mesenchymal migration to optimise their migration through 3D environments. This subtle migration phenotype has been underappreciated in the literature, with macrophages often being grouped and discussed together with other leukocytes, possibly due to the limitations of current chemotaxis assays. Transwell assays were originally designed in the 1960s but despite their long-known limitations, they are still one of the most popular methods of studying macrophage migration. This review aims to critically evaluate transwell assays, and other popular chemotaxis assays, comparing their advantages and limitations in macrophage migration studies.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Biology (Basel)
Año:
2020
Tipo del documento:
Article
País de afiliación:
Reino Unido