Morphology of enteric glia in colorectal carcinoma: A comparative study of tumor site and its proximal normal margin.
Morphologie
; 105(351): 267-274, 2021 Dec.
Article
en En
| MEDLINE
| ID: mdl-33309198
ABSTRACT
OBJECTIVE:
Colorectal carcinoma (CRC) is the third most common cancer in the world and fifth most common cancer in India. To understand the extent of perineural invasion (PNI) in CRC it is essential to study the morphology of enteric glial cells (EGCs). The aim of the study was to analyze the numerical density of EGCs and area of myenteric ganglia (MG) in the colonic tissue samples collected from CRC patients. MATERIAL ANDMETHODS:
Fifteen intraoperative tissue specimens were collected from the tumor site and 2cm proximal to the upper extent of tumor. The samples were divided into four groups group 1 (n=15) proximal tumor free colonic tissue; group 2 (n=3) well-differentiated; group 3 (n=8) moderately differentiated; group 4 (n=4) poorly differentiated adenocarcinoma. After processing the tissues were subjected to hematoxylin and eosin staining. The anti-S100ß and anti-GFAP antibodies were used to observe the EGCs.RESULTS:
In the H&E stained sections the number of myenteric ganglia appeared to be decreasing with increasing grade of adenocarcinoma. Immunostaining showed significant decreasing pattern in the numerical density of EGCs per myenteric ganglion and mean area of myenteric ganglia in relation to the thickness of circular muscle, corresponding to the increasing grades of adenocarcinoma. The morphology of the EGCs remained unaltered in the colonic tissue adjacent to the tumor site.CONCLUSION:
Significant loss of EGCs and neurodegeneration corresponded with the grade of tumor emphasizing on its prognostic value. The PNI was not seen in the clear margin proximal to the tumor site.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Colorrectales
/
Plexo Mientérico
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Morphologie
Asunto de la revista:
ANATOMIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
India