Your browser doesn't support javascript.
loading
The Microbiome of Leonardo da Vinci's Drawings: A Bio-Archive of Their History.
Piñar, Guadalupe; Sclocchi, Maria Carla; Pinzari, Flavia; Colaizzi, Piero; Graf, Alexandra; Sebastiani, Maria Letizia; Sterflinger, Katja.
Afiliación
  • Piñar G; Department of Biotechnology, Institute of Microbiology and Microbial Biotechnology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria.
  • Sclocchi MC; Laboratorio di Biologia, Istituto Centrale per la Patologia degli Archivi e del Libro (ICPAL), Rome, Italy.
  • Pinzari F; Institute for Biological Systems (ISB), Council of National Research of Italy (CNR), Monterotondo, Italy.
  • Colaizzi P; Laboratorio di Biologia, Istituto Centrale per la Patologia degli Archivi e del Libro (ICPAL), Rome, Italy.
  • Graf A; Applied Life Sciences/Bioengineering/Bioinformatics, FH Campus, Vienna, Austria.
  • Sebastiani ML; Laboratorio di Biologia, Istituto Centrale per la Patologia degli Archivi e del Libro (ICPAL), Rome, Italy.
  • Sterflinger K; Department of Biotechnology, Institute of Microbiology and Microbial Biotechnology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria.
Front Microbiol ; 11: 593401, 2020.
Article en En | MEDLINE | ID: mdl-33329475
ABSTRACT
Seven emblematic Leonardo da Vinci's drawings were investigated through third generation sequencing technology (Nanopore). In addition, SEM analyses were carried out to acquire photographic documentation and to infer the nature of the micro-objects removed from the surface of the drawings. The Nanopore generated microbiomes can be used as a "bio-archive" of the drawings, offering a kind of fingerprint for current and future biological comparisons. This information might help to create a biological catalog of the drawings (cataloging), a microbiome-fingerprint for each single analyzed drawing, as a reference dataset for future studies (monitoring) and last but not least a bio-archive of the history of each single object (added value). Results showed a relatively high contamination with human DNA and a surprising dominance of bacteria over fungi. However, it was possible to identify typical bacteria of the human microbiome, which are mere contaminants introduced by handling of the drawings as well as other microorganisms that seem to have been introduced through vectors, such as insects and their droppings, visible through the SEM analyses. All drawings showed very specific bio-archives, but a core microbiome of bacteria and fungi that are repeatedly found in this type of material as true degraders were identified, such as members of the phyla Proteobacteria, Actinobacteria, and Firmicutes among bacteria, and fungi belonging to the classes Sordariomycetes and Eurotiomycetes. In addition, some similarities were observed that could be influenced by their geographical location (Rome or Turin), indicating the influence of this factor and denoting the importance of environmental and storage conditions on the specific microbiomes.
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Front Microbiol Año: 2020 Tipo del documento: Article País de afiliación: Austria

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Front Microbiol Año: 2020 Tipo del documento: Article País de afiliación: Austria
...