Engineering an Optogenetic CRISPRi Platform for Improved Chemical Production.
ACS Synth Biol
; 10(1): 125-131, 2021 01 15.
Article
en En
| MEDLINE
| ID: mdl-33356154
ABSTRACT
Microbial synthesis of chemicals typically requires the redistribution of metabolic flux toward the synthesis of targeted products. Dynamic control is emerging as an effective approach for solving the hurdles mentioned above. As light could control the cell behavior in a spatial and temporal manner, the optogenetic-CRISPR interference (opto-CRISPRi) technique that allocates the metabolic resources according to different optical signal frequencies will enable bacteria to be controlled between the growth phase and the production stage. In this study, we applied a blue light-sensitive protein EL222 to regulate the expression of the dCpf1-mediated CRISPRi system that turns off the competitive pathways and redirects the metabolic flux toward the heterologous muconic acid synthesis in Escherichia coli. We found that the opto-CRISPRi system dynamically regulating the suppression of the central metabolism and competitive pathways could increase the muconic acid production by 130%. These results demonstrated that the opto-CRISPRi platform is an effective method for enhancing chemical synthesis with broad utilities.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Ácido Sórbico
/
Escherichia coli
/
Ingeniería Metabólica
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Optogenética
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Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas
Idioma:
En
Revista:
ACS Synth Biol
Año:
2021
Tipo del documento:
Article
País de afiliación:
China