Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility.
Science
; 371(6525)2021 01 08.
Article
en En
| MEDLINE
| ID: mdl-33414192
ABSTRACT
Posttranslational modifications of the microtubule cytoskeleton have emerged as key regulators of cellular functions, and their perturbations have been linked to a growing number of human pathologies. Tubulin glycylation modifies microtubules specifically in cilia and flagella, but its functional and mechanistic roles remain unclear. In this study, we generated a mouse model entirely lacking tubulin glycylation. Male mice were subfertile owing to aberrant beat patterns of their sperm flagella, which impeded the straight swimming of sperm cells. Using cryo-electron tomography, we showed that lack of glycylation caused abnormal conformations of the dynein arms within sperm axonemes, providing the structural basis for the observed dysfunction. Our findings reveal the importance of microtubule glycylation for controlled flagellar beating, directional sperm swimming, and male fertility.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
1_ASSA2030
Problema de salud:
1_doencas_nao_transmissiveis
Asunto principal:
Motilidad Espermática
/
Cola del Espermatozoide
/
Tubulina (Proteína)
/
Procesamiento Proteico-Postraduccional
/
Dineínas Axonemales
/
Fertilidad
/
Infertilidad Masculina
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Science
Año:
2021
Tipo del documento:
Article